vectashield vector laboratories cat  (Vector Laboratories)

Journal: Frontiers in Neuroscience

Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1

doi: 10.3389/fnins.2024.1436312

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Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI Vector Laboratories Cat#:H-1800.

Techniques: Dissection, shRNA, Control, Plasmid Preparation, Modification

Journal: Biomedicines

Article Title: Mitochondrial Genome-Encoded Long Noncoding RNA Cytochrome B (Lnc CytB ) and Mitochondrial Ribonucleases in Diabetic Retinopathy

doi: 10.3390/biomedicines12081637

Figure Lengend Snippet: Effect of high glucose on MRPP1 expression in retinal endothelial cells. ( a ) MRPP1 gene transcripts quantified by qRT-PCR using β-actin as a housekeeping gene. ( b ) Representative images showing MRPP1 mitochondrial localization using Alexa Fluor 488 (green)-conjugated and Texas Red (red)-conjugated secondary antibodies for MRPP1 and CoxIV, respectively. The line marker represents 10 μm. ( c ) AMI of MRPP1, calculated by quantifying the intensity of the green fluorescence. ( d ) Pearson’s correlation coefficient between MRPP1 and CoxIV, calculated using the colocalization software module. ( e ) Relative MRPP1 mRNA levels, quantified by qRT-PCR. Each measurement was made in duplicate/triplicate in 3–4 different cell preparations, and the values obtained for NG are considered as 1. The values are presented as means ± SDs. NG and HG = cells in 5 mM and 20 mM D-glucose, respectively; HG/ MRP , HG/ MRP -si, HG/EV and HG/SC = cells transfected with MRPP1 -overexpressing plasmids, MRPP1 -siRNA, an empty vector and control scrambled RNA, respectively, and incubated in 20 mM D-glucose; L-Gl = 20 mM L-glucose. * p < 0.05 compared to NG; # p < 0.05 compared to HG.

Article Snippet: To counterstain the nuclei, immunolabelled cells were mounted using 4′,6-diamidino-2-phenylindole (DAPI)-containing (blue) Vectashield mounting medium (cat. no. H-1000; Vector Laboratories).

Techniques: Expressing, Quantitative RT-PCR, Marker, Fluorescence, Software, Transfection, Plasmid Preparation, Control, Incubation

Journal: Biomedicines

Article Title: Mitochondrial Genome-Encoded Long Noncoding RNA Cytochrome B (Lnc CytB ) and Mitochondrial Ribonucleases in Diabetic Retinopathy

doi: 10.3390/biomedicines12081637

Figure Lengend Snippet: MRPP1–Lnc CytB interaction determined by RNA-FISH. ( a ) Representative RNA-FISH images with fluorescein 12-dUTP-labeled Lnc CytB probe in green and Texas Red-conjugated secondary antibody against MRPP1 in red (size marker = 10 μm). ( b ) Pearson correlation coefficient between Lnc CytB and MRPP1. ( c ) Arithmetic mean fluorescence intensity (AMI) of Lnc CytB . Each measurement was made in 5–7 cells and repeated in 2–3 different cell preparations. The values in the graphs are presented as means ± SDs. NG and HG = cells in 5 mM and 20 mM D-glucose, respectively; HG/ MRP , HG/ MRP-si , HG/EV and HG/SC = cells transfected with MRPP1 -overexpressing plasmids, MRPP1 -siRNA, an empty vector and scrambled RNA, respectively, and incubated in 20 mM D-glucose; L-Gl = 20 mM L-glucose. * p < 0.05 compared to NG; # p < 0.05 compared to HG.

Article Snippet: To counterstain the nuclei, immunolabelled cells were mounted using 4′,6-diamidino-2-phenylindole (DAPI)-containing (blue) Vectashield mounting medium (cat. no. H-1000; Vector Laboratories).

Techniques: Labeling, Marker, Fluorescence, Transfection, Plasmid Preparation, Incubation